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Registro Completo |
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Biblioteca(s): |
Embrapa Café; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais. |
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Data corrente: |
18/02/2011 |
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Data da última atualização: |
28/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIDAL, R. O.; MONDEGO, J. M. C.; POT, D.; AMBRÓSIO, A. B.; ANDRADE, A. C.; PEREIRA, L. F. P.; COLOMBO, C. A.; VIEIRA, L. G. E.; CARAZZOLLE, M. F.; PEREIRA, G. A. G. |
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Afiliação: |
RAMON OLIVEIRA VIDAL, UNICAMP/Instituto de Biologia; JORGE MAURÍCIO COSTA MONDEGO, Instituto Agronômico de Campinas; DAVID POT, Centre de Coopération Internationale en Recherche Agronomique pour le Développement; ALINNE BATISTA AMBRÓSIO, UNICAMP/Instituto de Biologia; ALAN CARVALHO ANDRADE, CENARGEN; LUIZ FILIPE PROTASIO PEREIRA, SAPC; CARLOS AUGUSTO COLOMBO, Instituto Agronômico de Campinas; LUIZ GONZAGA ESTEVES VIEIRA, Instituo Agronômico do Pará; MARCELO FALSARELLA CARAZZOLLE, UNICAMP/Instituto de Biologia; GONÇALO AMARANTE GUIMARÃES PEREIRA, UNICAMP/Instituto de Biologia. |
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Título: |
A high-throughput data mining of single nucleotide polymorphisms in Coffea species expresed sequence tags suggests differential homeologous gene expression in the allotetrapoloid Coffea arabica. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
PLANT PHYSIOLOGY, v. 154, p. 1053-1066. 2010. |
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Páginas: |
1053-1066 |
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Idioma: |
Inglês |
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Conteúdo: |
Polyploidization constitutes a common mode of evolution in flowering plants. This event provides the raw material for the divergence of function in homeologous genes, leading to phenotypic novelty that can contribute to the success of polyploids in nature or their selection for use in agriculture. Mounting evidence underlined the existence of homeologous expression biases in polyploid genomes; however, strategies to analyze such transcriptome regulation remained scarce. Important factors regarding homeologous expression biases remain to be explored, such as whether this phenomenon influences specific genes, how paralogs are affected by genome doubling, and what is the importance of the variability of homeologous expression bias to genotype differences. This study reports the expressed sequence tag assembly of the allopolyploid Coffea arabica and one of its direct ancestors, Coffea canephora. The assembly was used for the discovery of single nucleotide polymorphisms through the identification of high-quality discrepancies in overlapped expressed sequence tags and for gene expression information indirectly estimated by the transcript redundancy. Sequence diversity profiles were evaluated within C. arabica (Ca) and C. canephora (Cc) and used to deduce the transcript contribution of the Coffea eugenioides (Ce) ancestor. The assignment of the C. arabica haplotypes to the C. canephora (CaCc) or C. eugenioides (CaCe) ancestral genomes allowed us to analyze gene expression contributions of each subgenome in C. arabica. In silico data were validated by the quantitative polymerase chain reaction and allele-specific combination TaqMAMA-based method. The presence of differential expression of C. arabica homeologous genes and its implications in coffee gene expression, ontology, and physiology are discussed. MenosPolyploidization constitutes a common mode of evolution in flowering plants. This event provides the raw material for the divergence of function in homeologous genes, leading to phenotypic novelty that can contribute to the success of polyploids in nature or their selection for use in agriculture. Mounting evidence underlined the existence of homeologous expression biases in polyploid genomes; however, strategies to analyze such transcriptome regulation remained scarce. Important factors regarding homeologous expression biases remain to be explored, such as whether this phenomenon influences specific genes, how paralogs are affected by genome doubling, and what is the importance of the variability of homeologous expression bias to genotype differences. This study reports the expressed sequence tag assembly of the allopolyploid Coffea arabica and one of its direct ancestors, Coffea canephora. The assembly was used for the discovery of single nucleotide polymorphisms through the identification of high-quality discrepancies in overlapped expressed sequence tags and for gene expression information indirectly estimated by the transcript redundancy. Sequence diversity profiles were evaluated within C. arabica (Ca) and C. canephora (Cc) and used to deduce the transcript contribution of the Coffea eugenioides (Ce) ancestor. The assignment of the C. arabica haplotypes to the C. canephora (CaCc) or C. eugenioides (CaCe) ancestral genomes allowed us to analyze gene expression contribut... Mostrar Tudo |
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Thesagro: |
Coffea Arábica. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/29342/1/A-high-throughput.pdf
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Marc: |
LEADER 02657naa a2200253 a 4500
001 1917245
005 2023-02-28
008 2010 bl uuuu u00u1 u #d
100 1 $aVIDAL, R. O.
245 $aA high-throughput data mining of single nucleotide polymorphisms in Coffea species expresed sequence tags suggests differential homeologous gene expression in the allotetrapoloid Coffea arabica.$h[electronic resource]
260 $c2010
300 $a1053-1066
520 $aPolyploidization constitutes a common mode of evolution in flowering plants. This event provides the raw material for the divergence of function in homeologous genes, leading to phenotypic novelty that can contribute to the success of polyploids in nature or their selection for use in agriculture. Mounting evidence underlined the existence of homeologous expression biases in polyploid genomes; however, strategies to analyze such transcriptome regulation remained scarce. Important factors regarding homeologous expression biases remain to be explored, such as whether this phenomenon influences specific genes, how paralogs are affected by genome doubling, and what is the importance of the variability of homeologous expression bias to genotype differences. This study reports the expressed sequence tag assembly of the allopolyploid Coffea arabica and one of its direct ancestors, Coffea canephora. The assembly was used for the discovery of single nucleotide polymorphisms through the identification of high-quality discrepancies in overlapped expressed sequence tags and for gene expression information indirectly estimated by the transcript redundancy. Sequence diversity profiles were evaluated within C. arabica (Ca) and C. canephora (Cc) and used to deduce the transcript contribution of the Coffea eugenioides (Ce) ancestor. The assignment of the C. arabica haplotypes to the C. canephora (CaCc) or C. eugenioides (CaCe) ancestral genomes allowed us to analyze gene expression contributions of each subgenome in C. arabica. In silico data were validated by the quantitative polymerase chain reaction and allele-specific combination TaqMAMA-based method. The presence of differential expression of C. arabica homeologous genes and its implications in coffee gene expression, ontology, and physiology are discussed.
650 $aCoffea Arábica
700 1 $aMONDEGO, J. M. C.
700 1 $aPOT, D.
700 1 $aAMBRÓSIO, A. B.
700 1 $aANDRADE, A. C.
700 1 $aPEREIRA, L. F. P.
700 1 $aCOLOMBO, C. A.
700 1 $aVIEIRA, L. G. E.
700 1 $aCARAZZOLLE, M. F.
700 1 $aPEREIRA, G. A. G.
773 $tPLANT PHYSIOLOGY$gv. 154, p. 1053-1066. 2010.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
02/03/2020 |
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Data da última atualização: |
02/03/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
PANIZZI, A. R.; LUCINI, T. |
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Afiliação: |
ANTONIO RICARDO PANIZZI, CNPT; Tiago Lucini, bDepartamento de Zoologia, Universidade Federal do Paraná – UFPR, Av. Cel. Francisco H. dos Santos, 100, Jardim das Americas, CP 19020, CEP 81531-980, Curitiba, PR, Brasil. |
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Título: |
Body position of the stink bug Dichelops melacanthus (Dallas) during feeding from stems of maize seedlings. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Brazilian Journal of Biology, v. 79, n. 2, p. 304-310, 2019. |
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DOI: |
https://doi.org/10.1590/1519-6984.18250 |
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Idioma: |
Inglês |
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Conteúdo: |
The Neotropical green-belly stink bug, Dichelops melacanthus (Dallas) is a major pest of maize, Zea mays L. in the main production areas of Brazil. It usually feeds on the stems of young plants (seedlings) causing heavy damage by affecting the plant growth and mitigating seed yield. Laboratory studies were conducted to determine body position (upward or downward) of the bug on plant (seedling) stem during feeding and not feeding activities. Ten visual records were taken per day, each spaced one hour during 30 days of 10 adult bugs of similar age exposed to maize seedlings inside cages (plastic tubes). At each observation, it was recorded if the bug was feeding on the stem (i.e., stylets inserted into the plant tissue) or not, and its body position. During feeding, waveforms were recorded using the EPG (electropenetrography) technique, which were correlated with histological studies to reveal the feeding sites. Results indicated that when they were feeding, the majority of the bugs were in the downward position. In contrast, when the bugs were on the plants, and not feeding, they were mostly in the upward position. Waveforms generated using the EPG coupled with histological studies demonstrated that during ingestion bugs fed from the xylem vessels and from the parenchyma tissue using cell rupture strategy in the latter. No clear explanation was found to explain the preferred downward body position during ingestion, but some hypothesis are speculated. Keywords: Heteroptera, Pentatomidae, feeding behavior, EPG, plant histology. Posição do corpo do percevejo Dichelops melacanthus (Dallas) durante a alimentação em hastes de plântulas de milho O percevejo barriga-verde, Dichelops melacanthus (Dallas) é uma praga importante do milho, Zea mays L. nas principais áreas produtoras do Brasil. Usualmente alimenta-se nas hastes de plantas jovens (plântulas) causando danos severos no seu crescimento e reduzindo o rendimento de grãos. Estudos foram conduzidos em laboratório para determinar a posição do corpo (voltado para cima ou para baixo) dos percevejos nas hastes das plântulas de milho durante as atividades de alimentação e não-alimentação. Foram feitas dez observações por dia, espaçadas por uma hora, durante 30 dias em 10 percevejos adultos com idade semelhante sobre plântulas de milho colocadas em gaiolas (tubos de plástico). Em cada observação, anotou-se se o percevejo estava se alimentando (i.e., estiletes bucais inseridos no tecido vegetal) ou não, e a posição do corpo. Durante a alimentação, ondas eletromagnéticas foram registradas utilizando-se o EPG (técnica da eletropenetrografia), as quais foram correlacionadas com estudos histológicos para revelar os locais de alimentação. Os resultados indicaram que quando os percevejos estavam se alimentando, a maioria estava voltado para baixo. Em contraste, os percevejos sem se alimentar nas plântulas estavam a maioria voltados para cima. As ondas geradas pelo EPG junto com os estudos histológicos revelaram que os percevejos se alimentaram dos vasos do xilema e do tecido parenquimatoso usando a estratégia de ruptura celular no último. Não foi encontrada uma explicação clara para a preferência dos percevejos em se alimentarem na posição voltados para baixo, mas algumas hipóteses são especuladas. Palavras-chave: Heteroptera, Pentatomidae, comportamento alimentar, EPG, histologia da planta. MenosThe Neotropical green-belly stink bug, Dichelops melacanthus (Dallas) is a major pest of maize, Zea mays L. in the main production areas of Brazil. It usually feeds on the stems of young plants (seedlings) causing heavy damage by affecting the plant growth and mitigating seed yield. Laboratory studies were conducted to determine body position (upward or downward) of the bug on plant (seedling) stem during feeding and not feeding activities. Ten visual records were taken per day, each spaced one hour during 30 days of 10 adult bugs of similar age exposed to maize seedlings inside cages (plastic tubes). At each observation, it was recorded if the bug was feeding on the stem (i.e., stylets inserted into the plant tissue) or not, and its body position. During feeding, waveforms were recorded using the EPG (electropenetrography) technique, which were correlated with histological studies to reveal the feeding sites. Results indicated that when they were feeding, the majority of the bugs were in the downward position. In contrast, when the bugs were on the plants, and not feeding, they were mostly in the upward position. Waveforms generated using the EPG coupled with histological studies demonstrated that during ingestion bugs fed from the xylem vessels and from the parenchyma tissue using cell rupture strategy in the latter. No clear explanation was found to explain the preferred downward body position during ingestion, but some hypothesis are speculated. Keywords: Heteroptera, Pe... Mostrar Tudo |
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Palavras-Chave: |
EPG; Hastes de plântulas de milho; Maize; Neotropical green-belly stink bug; Percevejo barriga-verde; Plant histology; Stems of maize seedlings; Zea mays L. |
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Thesagro: |
Heteroptera; Milho. |
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Thesaurus NAL: |
Dichelops melacanthus; Feeding behavior; Pentatomidae. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/211324/1/1519-6984-bjb-1519-698418250.pdf
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Marc: |
LEADER 04363naa a2200301 a 4500
001 2120682
005 2020-03-02
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/1519-6984.18250$2DOI
100 1 $aPANIZZI, A. R.
245 $aBody position of the stink bug Dichelops melacanthus (Dallas) during feeding from stems of maize seedlings.$h[electronic resource]
260 $c2019
520 $aThe Neotropical green-belly stink bug, Dichelops melacanthus (Dallas) is a major pest of maize, Zea mays L. in the main production areas of Brazil. It usually feeds on the stems of young plants (seedlings) causing heavy damage by affecting the plant growth and mitigating seed yield. Laboratory studies were conducted to determine body position (upward or downward) of the bug on plant (seedling) stem during feeding and not feeding activities. Ten visual records were taken per day, each spaced one hour during 30 days of 10 adult bugs of similar age exposed to maize seedlings inside cages (plastic tubes). At each observation, it was recorded if the bug was feeding on the stem (i.e., stylets inserted into the plant tissue) or not, and its body position. During feeding, waveforms were recorded using the EPG (electropenetrography) technique, which were correlated with histological studies to reveal the feeding sites. Results indicated that when they were feeding, the majority of the bugs were in the downward position. In contrast, when the bugs were on the plants, and not feeding, they were mostly in the upward position. Waveforms generated using the EPG coupled with histological studies demonstrated that during ingestion bugs fed from the xylem vessels and from the parenchyma tissue using cell rupture strategy in the latter. No clear explanation was found to explain the preferred downward body position during ingestion, but some hypothesis are speculated. Keywords: Heteroptera, Pentatomidae, feeding behavior, EPG, plant histology. Posição do corpo do percevejo Dichelops melacanthus (Dallas) durante a alimentação em hastes de plântulas de milho O percevejo barriga-verde, Dichelops melacanthus (Dallas) é uma praga importante do milho, Zea mays L. nas principais áreas produtoras do Brasil. Usualmente alimenta-se nas hastes de plantas jovens (plântulas) causando danos severos no seu crescimento e reduzindo o rendimento de grãos. Estudos foram conduzidos em laboratório para determinar a posição do corpo (voltado para cima ou para baixo) dos percevejos nas hastes das plântulas de milho durante as atividades de alimentação e não-alimentação. Foram feitas dez observações por dia, espaçadas por uma hora, durante 30 dias em 10 percevejos adultos com idade semelhante sobre plântulas de milho colocadas em gaiolas (tubos de plástico). Em cada observação, anotou-se se o percevejo estava se alimentando (i.e., estiletes bucais inseridos no tecido vegetal) ou não, e a posição do corpo. Durante a alimentação, ondas eletromagnéticas foram registradas utilizando-se o EPG (técnica da eletropenetrografia), as quais foram correlacionadas com estudos histológicos para revelar os locais de alimentação. Os resultados indicaram que quando os percevejos estavam se alimentando, a maioria estava voltado para baixo. Em contraste, os percevejos sem se alimentar nas plântulas estavam a maioria voltados para cima. As ondas geradas pelo EPG junto com os estudos histológicos revelaram que os percevejos se alimentaram dos vasos do xilema e do tecido parenquimatoso usando a estratégia de ruptura celular no último. Não foi encontrada uma explicação clara para a preferência dos percevejos em se alimentarem na posição voltados para baixo, mas algumas hipóteses são especuladas. Palavras-chave: Heteroptera, Pentatomidae, comportamento alimentar, EPG, histologia da planta.
650 $aDichelops melacanthus
650 $aFeeding behavior
650 $aPentatomidae
650 $aHeteroptera
650 $aMilho
653 $aEPG
653 $aHastes de plântulas de milho
653 $aMaize
653 $aNeotropical green-belly stink bug
653 $aPercevejo barriga-verde
653 $aPlant histology
653 $aStems of maize seedlings
653 $aZea mays L
700 1 $aLUCINI, T.
773 $tBrazilian Journal of Biology$gv. 79, n. 2, p. 304-310, 2019.
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